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. 2020 May 5;10:7536. doi: 10.1038/s41598-020-64416-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neurotransmitters and their correlations. (A) Changes in brain metabolite concentrations in sham (black) and bile duct ligated (BDL) (grey) rats throughout the study, in absolute concentrations. (B) % difference in metabolites between BDL rats and shams at each time point. Left y-axis: % change. Right y-axis: change in mmol/kgww (kg of wet weight). (C) Correlations of glutamate with blood and brain measurements. *Comparison between shams and BDL for each time-point; *(grey) significance compared to week 2; % indicates significance compared to week 4; & significance compared to week 6.