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. 2020 May 5;10:7557. doi: 10.1038/s41598-020-64536-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Example of a BsAb purified using the DNA imbalance + CH1 purification method. (A) Analytical SEC chromatogram of the initial protein A eluate featuring a main peak comprised of target heterodimer and scFv-Fc homodimer in addition to a minor peak comprised of scFv-Fc half Ab. (B) Analytical SEC chromatogram of the CH1 eluate from (A) showing capture of only the desired heterodimeric BsAb. (C) Non-reduced GXII analysis of the protein A eluate (lane 1) and the CH1 eluate (lane 2). Captured species are indicated on the gel image.