Fig. 3. MCL-1^Matrix protects neurons and enhances mitochondrial bioenergetics in response to oxygen glucose deprivation.

a, b Representative Hoechst images showing healthy (uniformly labeled) and dead (condensed) nuclei in cortical neurons expressing GFP, MCL-1^Matrix or MCL-1^OM in response to OGD (a) and quantification of cell death is shown in (b) (averages ± SD of nine replicates from three independent experiments). c–e Cortical neurons expressing GFP, MCL-1^Matrix, and MCL-1^OM was treated with either normoxia or oxygen glucose deprivation (OGD) for 2 h and OCR was measured 24 h post injury (c). Quantification of ATP-linked (baseline OCR minus oligomycin-insensitive OCR) (d) and Maximal respiration capacity (FCCP-induced OCR) (e) (averages ± SD of 12 replicates from three independent experiments). f, g Total ATP levels (f) and ATP synthase-driven (Total ATP levels—oligomycin sensitive) ATP levels are shown in (g) (averages ± SD of nine replicates from three independent experiment Data information: one-way ANOVA followed by Tukey’s post hoc test.