Fig. 5. MCL-1^Matrix regulates Mitochondrial permeability transition pore through functional interactions with ATP synthase.

a Endogenous MCL-1 was immunoprecipitated from cortical neurons lysates and the eluted samples were analyzed for MCL-1 and ATP5A expression by western blot. b Cherry (control), Alpha, Beta, Gamma, Epsilon, and C subunit of ATP synthase constructs were transiently transfected into 293 T cells. Fourty-eight hours post-transfection, cells were lysed; immunoprecipitated with anti-flag beads and analyzed by western blot. c–e) Calcium retention capacity (CRC) in MCL-1 ∆/- MEFs expressing Cherry or MCL-1^Matrix and co-transduced with either ShControl or ShATP5G1 & G3. e Quantification of CRC shown in (c, d) (average ± SD from three independent experiments). Data information: one-way ANOVA followed by Tukey’s post hoc test.