Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 5;10:7525. doi: 10.1038/s41598-020-64571-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Anti-biofilm activity of the pitavastatin-fluconazole combination. The effect of the pitavastatin-fluconazole (FLC) combination was tested on the biofilm-forming ability of (a) C. albicans NR-29448, (b) C. glabrata HM-1123, and (c) C. auris 385, respectively. Fresh overnight cultures of the tested Candida strains were diluted 1:100 in RPMI 1640 medium. Cells were treated with pitavastatin (at 0.5 × MIC), fluconazole (FLC) at 2 µg/ml, or a combination of the two drugs, at the indicated concentration. Candida strains were incubated at 35 °C for 24 hours before discarding the non-adherent cells and staining the formed biofilms with 0.01% crystal violet. The absorbance of crystal violet-stained biofilms was measured at OD₅₉₅. *Indicates a significant difference between each treatment compared to the non-treated control. Whereas # indicates a significant difference between the tested pitavastatin-fluconazole combination relative to the single treatment with either fluconazole or pitavastatin. The statistical significance was considered for P < 0.05 as determined by one-way ANOVA with posthoc Dunnet’s test for multiple comparisons.