Figure 2:
Effect of lysing enzymes (A) concentration and (B) incubation time on C. sp. 2T2.1 transformation efficiency. (A) Varied concentrations of lysing enzymes were incubated for 60 min; (B) lysing enzymes (80 mg/ml) were incubated for varying times during protoplast formation. Error bars indicate standard deviation of three to five replicates.