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. 2020 May 5;10:7507. doi: 10.1038/s41598-020-64558-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tetranectin immunohistochemical tissue staining. Human cardiac tissue was obtained from cardiac by-pass patients, formalin-fixed and paraffin-embedded for immunostaining with either a rabbit anti-human monoclonal antibody against Tetranectin or an IgG isotype control antibody. Sections were counterstained with Haematoxylin and imaged using Aperio ScanScope digital scanner. A positive pixel count algorithm was applied to analyse the area and intensity of immunoreactivity. Representative images presented; IgG control (a); examples of Tetranectin (brown) and haematoxylin (blue) stained tissue sections (b,c); Example of the Tetranectin positive pixel algorithm used to quantify tissue protein expression (d). Images were captured at 20x magnification.