Fig. 9.

Effect of 2-week withdrawal from cocaine exposure during adulthood on mBDNF and trkB protein levels in the PrhC.

Rats were treated with cocaine or saline from PND 63 to PND 77. Two weeks after the end of treatment (PND 91), rats were tested for NOR and sacrificed immediately after the test. mBDNF protein levels (14 kDa) are shown in the whole homogenate (panel a) and in the post-synaptic density fraction (panel b) of the PrhC. TrkB protein levels (145 kDa) are shown in the whole homogenate (panel c) and in the post-synaptic density fraction (panel d) of the PrhC. In the upper panel, representative immunoblots are shown for mBDNF, trkB and β-Actin proteins in the homogenate and PSD of PrhC. Results are expressed as percentages of saline-treated rats not exposed to NOR (saline-no test). Histograms represent the mean ± SEM of five to seven rats per group. *p < 0.05, **p < 0.01 vs. saline-no test rats; ^#p < 0.05, ^###p < 0.001 vs. cocaine- no test (two-way ANOVA followed by Tukey HSD test).

S = saline-no test; S/T = saline-test; C = cocaine-no test; C/T = cocaine-test.