Table 2:

Formation of amplicon and primer dimer in the presence of target and no target with a “worst case” pair of primers

	Ct of amplicon or primer dimer formation		Average ΔCt
Primer types	With target (104 copies)	Without target (NTC)	Ct (NTC) − Ct (target)
Std primers	7.6a	6.0a	−1.6
2SAMRS-1N	22.7	20.9	−1.8
3SAMRS-1N	27.8	44.5	16.7
4SAMRS-1N	27.2	37.7	10.5
4SAMRS-2N	26.6	35.0	8.4
4SAMRS-3N	20.8	20.1	−0.7
4SAMRS-4N	18.8	18.1	−0.7

^a Based on melting temperature (Fig. 3, right), these are all primer dimers at 3 mM of Mg²⁺.