Table 3:
Ct and ΔCt values of PCR amplifications using reverse primer with different numbers of SAMRS components with KlenTaq1 DNA polymerase
| Allele-specific PCR using KlenTaq1 DNA polymerase | ||||
|---|---|---|---|---|
| Forward primer | Reverse primer | Match C:G (ΔCt) | Mismatch C:A (ΔCt) | NTC (ΔCt) |
| Common HIV-Std-Fp | HIV-Std-Rp | 29.8 (0) reference | 32.1 (2.3) | 36.1 (6.3) |
| Std-1Mis C to T | 30.1 (0.3) | 39.8 (dimer) | 36.7 (6.9) | |
| 1SAMRS-Tc-1N | 29.8 (0) | 34.6 (4.8) | 37.0 (7.2) | |
| 2SAMRS-tc-1N | 30.1 (0.3) | 35.4 (5.6) | 42.9 (13.1)a | |
| 3SAMRS-atc-1N | 31.1 (1.3) | 40.2 (10.4) | 55 (25.2)a | |
| 4SAMRS-gatc-1N | 32.9 (3.1) | 42.9 (13.1) | NA | |
| 2SAMRS-aTc-1N | 30.5 (0.7) | 37.2 (7.4) | 53.9 (24.1)a | |
| 3SAMRS-gaTc-1N | 31.7 (1.9) | 38.8 (9) | NA | |