Table 4:
Ct and ΔCt values of PCR amplifications using reverse primer with different numbers of SAMRS components with HiDi DNA polymerase
| Allele-specific PCR using HiDi DNA polymerase | ||||
|---|---|---|---|---|
| Forward primer | Reverse primer | Match C:G (ΔCt) | Mismatch C:A (ΔCt) | NTC (ΔCt) |
| Common HIV-Std-Fp | HIV-Std-Rp | 29.7 (0) reference | 37.4 (7.7) | NA |
| Std-1Mis (C to T) | 33.8 (4.1) | 37.0 (dimer)a | 49.0 (19.3) | |
| 1SAMRS-Tc-1N | 28.8 (−0.9) | 55.8 (26.1) | NA | |
| 2SAMRS-tc-1N | 30.6 (0.9) | 51.5 (21.8) | NA | |
| 3SAMRS-atc-1N | 33.1 (3.4) | NA | NA | |
| 4SAMRS-gatc-1N | 36.4 (6.7) | NA | NA | |
A “least-worst case” pair of primers was tested in allele-specific PCR (60 cycles). All PCR have three replicates for matched template G, mismatched template A, and NTC.
aOne-third of replicates show amplification signal. Dimer indicates primer dimer. NA indicates No Amplification.