Table 5:
Ct and ΔCt values of PCR amplifications using reverse primer with different numbers and positions of SAMRS components with KlenTaq1 DNA polymerase
| Ct and ΔCt of SNP discrimination using KlenTaq1 DNA polymerase | |||||||
|---|---|---|---|---|---|---|---|
| Forward primer | Reverse primer | Match T:A | Mismatch T:G | Mismatch T:C | Mismatch T:T | Average (ΔCt) | NTC |
| Common Std-Fp | Std-Rp-T-allele | 28.3 (0) reference | 31.3 (3) | 33.1 (4.8) | 33.5 (5.2) | 4.4 | NA |
| 2SAMRS-Tgt-1N | 29.5 (1.2) | 34.5 (6.2) | 37.3 (9) | 37.2 (8.9) | 8.1 | NA | |
| 3SAMRS-tgt-1N | 29.9 (1.6) | 36.1 (7.8) | 38.5 (10.2) | 38.6 (10.3) | 9.5 | NA | |
| 4SAMRS-ctgt-1N | 29.7 (1.4) | 37.7 (9.4) | 45.5 (17.2) | 38.8 (10.5) | 12.4 | NA | |
| 4SAMRS-actg-2N | 30.1 (1.8) | 38.5 (10.2) | 42.1 (13.8) | 40.1 (11.8) | 12.0 | NA | |
| 4SAMRS-tact-3N | 29.6 (1.3) | 36.0 (7.7) | 38.6 (10.3) | 37.7 (9.4) | 9.2 | NA | |
| 4SAMRS-ttac-4N | 29.5 (1.2) | 35.0 (6.7) | 37.1 (8.8) | 38.1 (9.8) | 8.4 | NA | |