Table 7:
Compare PCR efficiency and sensitivity of standard primers with SAMRS primers
| Allele-specific PCR using HiDi DNA polymerase | ||||||||
|---|---|---|---|---|---|---|---|---|
| Matched or mismatched template |
104 copies |
103 copies |
102 copies |
NTC | ||||
| Forward primer | Reverse-T-allele | Match T:A | Mismatch T:G | Match T:A | Mismatch T:G | Match T:A | Mismatch T:G | |
| Std-Fp | Std-Rp | 27.5 (0.0) | 35.8 (8.3) | 32.0 (4.5) | 38.9 (11.4) | 34.8 (7.3) | 50.6 (23.1)b | 38.6 (dimer)c |
| 2SAMRS-Fp | 2SAMRS-Rp | 29.5 (2.0) | 39.9 (12.4) | 33.8 (6.3) | 42.9 (15.4) | 37.7 (10.2) | 55 (27.5)b | 42.5 (dimer)a |
All allele-specific PCR have six replicates for matched template A, mismatched templates G, and NTC.
aOne-sixth,
btwo-sixths, and
cthree-sixths of replicates generated amplification signal. Dimer indicates primer dimer.