Figure 5.

Identification of Common Binding Sites in the 10 Genes Most Strongly Downregulated by Both STOX1A and B in BeWo Cells, with or without Induction of Fusion by Forskolin

(A) The 10 genes were identified from the microarray analysis by screening for profiles similar to ANXA1.

(B) MEME analysis of the 10 promoter regions (~5,000 bp) revealed common motifs in nine of them. The two most significantly enriched motifs were generally found in common (light blue and red).

(C) A biotinylated probe was synthetized for gel-shift experiments. A STRE2 element was found in the red part of the motif and is underlined in purple.