Table 2.

Discharge parameters behaviour of different cycles of CLWW treatment (1500.00 CU) with P. pastoris X33/pGAPZαA-LaccPost-Stop (Clone 1) immobilized of the cells

Discharge parameter	0 h	74 h	Removal (%)
Cycle 1
TOC (mg L−1)	2214.26 ± 46.84	591.97 ± 3.63	73.26 ± 0.40
TC (mg L−1)	2290.00 ± 48.08	608.50 ± 4.38	73.42 ± 0.37
IC (mg L−1)	75.74 ± 1.24	16.53 ± 0.75	78.18 ± 0.64
TN (mg L−1)	411.49 ± 20.53	264.40 ± 4.81	36.49 ± 5.00
CU	1500.00 ± 0.00	12.63 ± 2.19	99.16 ± 0.15a
COD (mg L−1)	6045.00 ± 35.36	1660.00 ± 0.00	72.43 ± 0.00
BOD5 (mg L−1)	999.00 ± 0.00	999.00 ± 0.00	0.00 ± 0.00
TSS (mg L−1)	275.00 ± 35.36	50.00 ± 0.00	80.00 ± 0.00
BOD5/COD	0.16	0.60	N/A
TOC/TN	5.38	2.24	N/A
COD/TOC	2.73	2.80	N/A
pH	4.16 ± 0.20	5.05 ± 0.20	N/A
Cycle 2
TOC (mg L−1)	135.86 ± 6.70	74.01 ± 0.50	45.45 ± 3.06
TC (mg L−1)	165.09 ± 3.97	93.22 ± 1.22	43.36 ± 2.65
IC (mg L−1)	29.11 ± 0.93	19.36 ± 0.57	33.49 ± 0.19
TN (mg L−1)	94.76 ± 1.63	93.20 ± 0.62	2.17 ± 1.32
CU	1500.00 ± 0.00	6.31 ± 4.37	99.58 ± 0.29a
COD (mg L−1)	760.00 ± 155.56	280.00 ± 14.14	67.82 ± 1.63
BOD5 (mg L−1)	999.00 ± 0.00	250.00 ± 0.00	74.98 ± 0.00
TSS (mg L−1)	66.67 ± 28.87	66.67 ± 28.87	0.00 ± 0.00
BOD5/COD	1.30	0.80	N/A
TOC/TN	1.43	0.79	N/A
COD/TOC	5.59	3.78	N/A
pH	7.21 ± 0.20	8.01 ± 0.20	N/A
Cycle 3
TOC (mg L−1)	47.08 ± 1.41	20.60 ± 3.13	56.13 ± 7.96
TC (mg L−1)	54.22 ± 2.17	27.81 ± 1.54	48.61 ± 4.59
IC (mg L−1)	6.33 ± 0.73	7.34 ± 0.85	0.00 ± 0.00
TN (mg L−1)	60.07 ± 0.66	60.60 ± 0.00	0.00 ± 0.00
CU	1500.00 ± 0.00	10.10 ± 2.19	99.33 ± 0.15a
COD (mg L−1)	370.00 ± 0.00	295.00 ± 21.21	15.71 ± 6.06
BOD5 (mg L−1)	250.00 ± 0.00	250.00 ± 0.00	0.00 ± 0.00
TSS (mg L−1)	166.67 ± 28.87	33.33 ± 28.87	83.33 ± 14.43
BOD5/COD	0.60	0.80	N/A
TOC/TN	0.78	0.34	N/A
COD/TOC	7.86	14.32	N/A
pH	7.28 ± 0.20	7.50 ± 0.20	N/A

N/A does not apply, N/C unable to calculate

^aPercentage calculated from 1500.00 CU at the beginning of treatment