Figure 3.

Best model fit of the DV replication model (Huh7) and the coupled model (A549) compared to experimental data measured in Huh7 and A549 cells (for parameter values see Tables S6, S7). Experimental measurements are represented as mean μ± standard deviation σ. The DV replication model and the coupled model were fitted simultaneously to the Huh7 and A549 data sets with cell line specific differences mediated by host factors, the antiviral HIR effect, and DV countermeasures (Equations 1 to 28). (A) shows the model fit of luciferase compared to the luciferase measurements (L = Luc), (B) model fit of total (+)RNA to the (+)RNA measurements ($R_P^{tot}$ = (+)RNA), (C) model fit of extracellular virus to its measurements (V^tot= Virus), (D) model fit compared to measurements of the HIR ($I_R^{tot}=$ ISG mRNA and F_EX = extracellular IFN), while (E) shows the model prediction of the coupled model with cell line specific Huh7 parameter values and the knocked-out RIG-I pathway activation $k_{rig}=0h^{-1}$ compared to IFN measured in Huh7 cells.