Figure 2.

Course of P. chabaudi challenge infection in BALB/c mice immunized with dead B. microti. BALB/c mice were immunized three times at 2-weeks intervals with either glutaraldehyde-fixed B. microti-pRBCs or glutaraldehyde-fixed non-parasitized RBCs (npRBCs). Two weeks after the last immunization, all mice were challenge-infected with P. chabaudi. Parasitemia (A), hematocrit (B), body weight (C), and survival rates (D) were monitored over a period of 28 days after challenge infection. Results are expressed as mean values ± the SD of five mice. Mean percent parasitemia, hematocrit, and body weight values are calculated from individual values taken from all surviving mice at each specific time point. Data presented are from one experiment representative of three.