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. 2020 Apr 29;11:454. doi: 10.3389/fphar.2020.00454

Figure 3.

Figure 3

Maternal pentaerythritol tetranitrate (PETN) treatment leads to epigenetic changes in the kidney of female offspring. Parental spontaneously hypertensive rats (SHR) were treated with or without PETN (50 mg/kg/day) during pregnancy and lactation periods. (A) The chromatin accessibility at the proximal promotor regions around the transcription start site of ACE, FGF2, and NHE3 was studied by MNase digestion. Histone 3 lysine 4 trimethylation (H3K4me3), histone 3 lysine 9 trimethylation (H3K9me3), and histone 3 lysine 27 trimethylation (H3K27me3) at the proximal promotor regions around the transcription start site of angiotensin-converting enzyme (ACE) (B), fibroblast growth factor (FGF2) (C) and sodium-hydrogen antiporter 3 (NHE3) (D) were studied with chromatin immunoprecipitation (ChIP) followed by quantitative PCR using kidney of 16-week-old offspring. Nonspecific IgG was used as negative control of the ChIP experiment. Column represents mean ± SEM, n= 6. Student’s t test was used for comparison of PETN group with control group. Student’s t test was used for comparison of PETN group with control group. *P < 0.05; **P < 0.01 vs. control group.