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. 2020 Apr 30;14:1651–1662. doi: 10.2147/DDDT.S249162

Figure 6.

Figure 6

Effect of astaxanthin on P2X7R, IL-1β, Cox-2 and ATP expression in LPS-induced rat microglia. (A) The mRNA expression of IL-1β and Cox-2 at different concentrations of LPS. (B, C) The mRNA expression of IL-1β and Cox-2 treated with different concentrations of AST. (D) WB analysis for P2X7R, IL-1β and Cox-2 protein levels in the rat microglia. (E) The expression levels of P2X7R, IL-1β and Cox-2 proteins in microglia. (F) The mRNA expression of P2X7R, IL-1β and Cox-2 were examined by RT-PCR. (G) The concentration of extracellular ATP (H) The concentration of intracellular ATP. The relative expression of protein and mRNA were normalized to β-actin expression. The concentration of extracellular and intracellular ATP was measured by ELISA kit. Cells were treated with designed concentration of AST (50μM) and LPS (10μg/mL) for 48h. Values are presented as the mean ± SD (*p < 0.05, **p < 0.01 vs Control group; #p < 0.05, ##p < 0.01 vs LPS group).