Table 1.
Nucleotide sequences of forward and reverse primers used for qPCR.
| Gene | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) | Amplicon length (bp) | Source |
|---|---|---|---|---|
| elf1a | CTGGAGGCCAGCTCAAACAT | TCAAGAAGAGTAGTACCGCTAGCATTAC | 85 | NM_131263.1 |
| rpl13 | TCTGGAGGACTGTAAGAGGTATGC | AGACGCACAATCTTGAGAGCAG | 147 | NM_212784 |
| cldn5a | CTCGGGGGAAGCATACTCTG | TATTCCCCGTTGGATGTGGC | 101 | NM_213274.1 |
| cldn2 | CCCCTACTGGGCAACATCTG | CTTGCAGGTCGACGGTTAGT | 155 | XM_005170272.4 |
| Oclnb | TGACAAAGTCAGTGCTGCTCA | GGATATTCATCTGTGTCAAGCTCAT | 162 | NM_001008618.1 |
| tlr2 | TGCTGTCGGTCGATTACCTG | ACACAGGGAAAACGAAGGCT | 130 | NM_212812.1 |
| tlr4ba | ACTTTCAAGATTTCCGTTGAGGTAT | CCACAAGAACAAGCCTTTGCAG | 209 | NM_001131051.1 |
| tlr4bb | ATCACACTGCTGTGTTGCGA | AGGCCAGTGAGAGCTTTGTG | 178 | NM_212813.2 |
| tlr5a | TGGTTCCGGTTGTGAGTTCC | TCTTGAAGATGACACCGCGT | 162 | XM_001919017.6 |
| tlr5b | GGTCTTGAAGCGAGTGGTGT | CATTTTGCGCCAAGGTCAGT | 118 | NM_001130595.2 |
| il1β | GATGGCATGCGGGCAATATG | AGCTCATTGCAAGCGGATCT | 116 | NM_212844.2 |
| il10 | GACCATTCTGCCAACAGCTC | ACCATATCCCGCTTGAGTTCC | 102 | NM_001020785.2 |
| myd88 | GTTTGCGCTCAGTCTTTGCC | GGTCAGAAAGCGCAGAATGC | 103 | NM_212814.2 |
| cxcr4a | ACTTGTACAGCAGCGTCCTC | CTTTGGCGAACACCAAGTCG | 174 | NM_131882.3 |
| cxcr4b | GCGCCTTTTTGAGCACACTT | ATTGCTGACTGAGAGGTCGC | 127 | NM_131834.1 |
| ptpn6 | ACAGGAAGAACTGCGCTCAT | TGCAGCACAGCCAAGTAGAT | 225 | NM_199960.1 |
Reference genes: elongation factor 1α (elf1α); ribosomal protein L13 (rpl13).