Table 1. All primer sequences used for RT-PCR and RT-qPCR.
| Genes | Primer sequences (5'-3') | Length (bp) | GenBank Acc No. | Annealing temp (°C) |
|---|---|---|---|---|
| Xbp1 | F-GGCAGAGACCAAGGGGAATG | 263 | FJ213449.1 | 60 |
| R-GGGTCGACTTCTGGGAGCTG | ||||
| BiP | F-ACCAATGACCAAAATCGCCT | 246 | J03214.1 | 60 |
| R-GTGACTTTCCAGCCACTCAA | ||||
| GRP94 | F-CTGCTGAAGGGGAA TTACC | 197 | Y09136.1 | 60 |
| R-ATCATCTGAGTCCACAACGC | ||||
| ATF4 | F-TGAGCCCTGACTCCTATCTG | 277 | NM_001123078.1 | 60 |
| R-TCCAGCTCTTTACATTCGCC | ||||
| CHOP(DDIT3) | F-AAGACCCAGGAAACGGAAAC | 261 | NM_001144845.1 | 60 |
| R-TCCAGGAAAGGTCAGCAGTA | ||||
| Nrf2 (NFE2L2) | F-CCCATTCACAAAAGACAAACATTC | 72 | GU991000.1 | 60 |
| R-GCTTTTGCCCTTAGCTCATCTC | ||||
| HO-1 (HMOX1) | F-CACTCACAGCCCAACAGCA | 162 | NM_001004027.1 | 55 |
| R-GTGGTACAAGGACGCCATCA | ||||
| Bax | F-ACTGGACAGTAACATGGAGC | 294 | XM003127290.3 | 55 |
| R-GTCCCAAAGTAGGAGAGGAG | ||||
| Caspase-3 | F-GAGGCAGACTTCTTGTATGC | 237 | NM_214131 | 60 |
| R-CATGGACACAATACATGGAA | ||||
| GAPDH | F-GGGCATGAACCATGAGAAGT | 230 | AF017079 | 58 |
| R-AAGCAGGGATGATGTTCTGG |
Xbp1, X-box binding protein 1; BiP, binding protein; GRP94, glucose-regulated protein 94; ATF4, activating transcription factor 4; CHOP, C/EBP homologous protein; Nrf2, nuclear factor 2; HO-1, hemeoxygenase 1 GAPDH, glyceraldehyde 3-phosphate dehydrogenase.