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. 2020 Apr 29;11:431. doi: 10.3389/fpls.2020.00431

FIGURE 3.

FIGURE 3

Antioxidant and photosynthetic inhibitors mitigate GCN2 kinase activation under cold and salt stress. (A) Time course of eIF2α phosphorylation in Wt seedlings grown on medium supplemented with 0.5 mM ascorbate and reduced glutathione for 10 days and shifted to 150 mM NaCl with either antioxidants (Asc + GSH) or mock control. Seedlings were transferred at ZT2 and harvested at 0, 10, 30, and 120 min. The graph shows eIF2α phosphorylation signals from three independent experiments with average, individual data points, and standard deviations. (B,C) eIF2α phosphorylation in Wt seedlings treated with either DMSO control (Mock), 8 μM of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), or 16 μM of 2,5-Dibromo-6-isopropyl-3-methyl-1,4-benzoquinone (DBMIB) 30 min prior to treatment for 2 h with (B) 150 mM NaCl or (C) 4°C cold. Welch’s unpaired t-test P-values for comparisons against NaCl/cold were *** < 0.001, ** < 0.01. For details see legend to Figures 1, 2.