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. 2020 Apr 29;11:431. doi: 10.3389/fpls.2020.00431

FIGURE 4.

FIGURE 4

Loss of GCN2 renders increased sensitivity toward cold stress. (A) Top: Representative images of 3-day-old Wt (Ler) and gcn2-1 mutant seedlings grown under a 16 h light/8 h dark cycle (long day, LD) at 22°C. Seedlings were grown on medium with 0.1% sucrose for 3 days and transferred to no sucrose (day 0). Bottom: Same seedlings after 30 days of LD cycle at 4°C. Scale bars are 10 mm. (B) Primary root length of Wt and gcn2-1 mutant seedlings from (A). Error bars indicate standard error of the mean from four biological replicates with n > 80 per experiment (Welch’s t-test *P < 0.05).