FIGURE 5.

gcn2 mutants are more sensitive to salt stress. (A) Wt (Ler) and gcn2-1 mutant seedlings were grown under a 16 h light/8 h dark cycle for 3 days on plant medium supplemented with 0.1% sucrose. On day 3, seedlings were transferred to new plates with 0.1% sucrose and 150 mM NaCl (salt treatment), 300 mM mannitol (osmotic control), or no supplement (mock). Root growth was recorded for another 9 days. Scale bar is 10 mm. Seedlings that bleached out and died are indicated by asterisks. (B) Primary root length of Wt and gcn2-1 mutants from (A), excluding dead seedlings. Error bars indicate standard error of the mean of four biological replicates with n > 36 per experiment (Welch’s t-test *P < 0.05; **P < 0.005).