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. 2020 Apr 29;11:431. doi: 10.3389/fpls.2020.00431

FIGURE 6.

FIGURE 6

gcn2 mutants accumulate less fresh weight and exhibit low survival under salt stress. (A) Left panel: Fresh weight (grams) of Wt(Ler) and gcn2-1 mutant seedlings after 9 days of growth on 300 mM mannitol or 150 mM NaCl, or no supplement (Sucrose). The medium for all seedlings contained 0.1% sucrose. Right panel: Percent survival of Wt and gcn2-1 mutant seedlings at days 6 and 9 on 150 mM NaCl. Data include the experiment in Figure 5. (B) Fresh weight and percent survival of wild-type Columbia [Wt (Col)] and gcn2-2 mutant (gcn2-2) seedlings at days 6 and 9 on 150 mM NaCl. Data include the experiment in Supplementary Figure 4. Error bars indicate standard error of the mean of four biological replicates with n > 36 per experiment (Welch’s t-test *P < 0.05; **P < 0.005).