FIGURE 7.

Ribosome-RNA profile of wild-type and gcn2-1 under standard growth conditions and cold stress. Top: (A,B) Schematic of light regimen showing seedling growth in long-day period (16 h light and 8 h dark) indicating the beginning of cold (4°C) treatment starting at ZT2 (blue arrow) and the sampling time at ZT4 (red arrow). Bottom: UV absorbance profile at 254 nm of 14-day-old wild-type Landsberg [Wt(ler)] and gcn2-1 mutant (gcn2-1) seedlings at (A) 22°C at ZT4 (Mock) or subjected to cold at 4°C (B) for 2 h, or (C) for 24 h under a long-day period. The positions of the 40S, 60S, 80S, and the polysomes are indicated on the profiles. The ratio of polysomes (P) to monosomes (M) is indicated with standard error from three replicates.