Figure 1.
(A) The parametric unit cell of the computer-aided design of the porous Ti scaffolds, which consists entirely of identical beams with constant circular cross sections (0.1 mm) and a beam length of 0.9 mm. (B) A typical image of a selective laser melting produced Ti scaffold. (C) An image of the in-house developed perfusion bioreactor equipped with parallel perfusion circuits. (D) Schematic representation of the bioreactor setup used for three-dimensional (3D) dynamic culture, consisting of a medium reservoir containing 10 mL of medium, a peristaltic pump forcing the culture medium through the porous scaffold that was positioned in the perfusion chamber. (E) Schematic representation of simulated neotissue growth inside scaffold with growth velocity a function of curvature (κ), flow induced shear stress (τ), oxygen concentration (Co), glucose concentration (Cg), and pH. Adapted from Papantoniou et al. (2014b) and Guyot et al. (2015).