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. 2020 Apr 28;11:754. doi: 10.3389/fmicb.2020.00754

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Copyright © 2020 Raruang, Omolehin, Hu, Wei, Han, Rajasekaran, Cary, Wang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determining the presence and level of expression of the target gene in the T0 transgenic leaf tissues. (A) PCR confirmation of the presence (+) or absence (−) of target gene in versicolorin (ver-1, aflM) RNAi vector transformed T0 leaf tissues. AflM RNAi plasmid DNA was used as a positive control and the genomic DNA from commercial maize line B104 was used as a negative (−) control. (B) Expression of transgene aflM in the T0 leaf tissue of various transformation events relative to 18S rRNA using real time PCR. AflM11 is negative for the transgene.