Figure 2.

Upregulation of circ_001653 Is Identified in NP Tissues of IDD

The primary cells observed under the microscope at the 3^rd day (200×) (A). The primary cells under the microscope at the 20^th day (200×) (B). The cells at the first passage observed under the microscope at the 2^nd day (200×) (C). The immunofluorescence staining of COL2A1 (400×) (D). The immunofluorescence staining of aggrecan (400×) (E). The positive expression of marker CD24 measured by flow cytometry (F). The relative expression of circ_001653 in SCI tissues and degenerative NP tissue measured by qRT-PCR (G). The correlation between circ_001653 and severity of IDD assessed by Pearson’s correlation analysis (H). *p < 0.05 versus the SCI tissues. Statistical data were measurement data described as mean ± standard deviation; n = 30 for the SCI tissues, and n = 36 for the NP tissues from the IDD patients. Data between two groups were analyzed by unpaired Student’s t test.