Algorithm 1: DRAGON
INPUT PCA components derived from highly variable genes
PARAMETERS Set maximum number of clusters (K), vector of temperatures (T), number of iterations (Z), convergence criterion
1	FOR T in initial to final temperature
2	IF T>0
3	IF Number of clusters < Maximum clusters
4	Split clusters with 2λ > T
5	FOR Z iterations or convergence
6	Expectation
7	Maximization
8	END
9	IF T=0
10	FOR Z iterations or convergence
11	Expectation
12	Maximization
13	END
14	END