Table 2.
Univariate Analysis of Factors Associated With Detectable Neutralizing Antibody Titers Against Toxin B in Children With Toxigenic Clostridioides difficile Colonization for at Least 1 Month’s Duration
| Characteristic | Detectable Toxin B NAb (n = 5) | Nondetectable Toxin B NAb (n = 7) | P Value |
|---|---|---|---|
| Male sex, no. (%) | 3 (60) | 6 (86) | .52 |
| Hispanic or Latino ethnicity, no. (%) | 2 (40) | 4 (57) | 1 |
| Breastfeeding, no. (%) | 2 (40) | 6 (86) | .22 |
| Stopped breastfeeding before study blood draw, no. (%) | 2/2 (100) | 5/6 (83) | 1 |
| Median age stopped breastfeeding, mo | 7 | 3 | .11 |
| Median age at first detected TCD colonization, mo | 4 | 4 | .75 |
| Median duration of detectable TCD colonization, mo | 5 | 5 | .62 |
| Median stool tcdB PCR cycle thresholda | 23.9 | 25.4 | .42 |
| At least 1 toxin EIA-positive stool, no. (%) | 4 (80) | 5 (71) | 1 |
| Median antitoxin B IgG (AU) | 43.0 | 7.8 | .09 |
| Median antitoxin B IgA (AU) | 2.4 | 0.9 | .02 |
N = 12.
Abbreviations: AU, arbitrary ELISA units; EIA, enzyme immunoassay; Ig, immunoglobulin; IQR, interquartile range; Nab, neutralizing antibody; NTCD, nontoxigenic Clostridioides difficile; PCR, polymerase chain reaction; TCD, toxigenic Clostridioides difficile.
aThe single lowest stool tcdB PCR cycle threshold value measured per patient included in this analysis.