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. 2020 Jan 14;2020:7430968. doi: 10.1155/2020/7430968

Figure 1.

Figure 1

In vitro aged hUC-MSCs exhibit reduced proliferative and migratory capacities along with decreased c-Jun expression. (a) The proliferative capacity of hUC-MSCs at passages 5, 10, and 15 determined with the CCK-8 assay. (b) The migratory capacity of hUC-MSCs at passages 5 and 15 evaluated with the in vitro scratch assay. (c, d) The relative c-Jun mRNA (c) and protein (d) levels in hUC-MSCs at passages 5, 10, and 15 by qRT-PCR and western blot analysis, respectively. n = 4, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.