Table 1.
Candidate reference genes and characteristics of RT-qPCR primers.
| Gene symbol | Gene name | Accession no. | Primer sequence | Amplicon size | Efficiency (%) | R 2 |
|---|---|---|---|---|---|---|
| Actb | Beta-actin | NM_007393.5 | F: CCTTCTTGGGTATGGAATCCTGT R: ACTGTGTTGGCATAGAGGTCTTTAC |
101 | 95.9 | 0.990 |
| β2m | Beta-2 microglobulin | NM_009735.3 | F: CATGGCTCGCTCGGTGAC R: CAGTTCAGTATGTTCGGCTTCC |
135 | 96.8 | 0.995 |
| Tuba | Alpha-tubulin | NM_011653.2 | F: TGTCCTGGACAGGATTCGC R: CTCCATCAGCAGGGAGGTG |
115 | 95.0 | 0.997 |
| Gusb | Beta-glucuronidase | NM_010368.1 | F: CCGACCTCTCGAACAACCG R: GCTTCCCGTTCATACCACACC |
169 | 96.8 | 0.993 |
| Gapdh | Glyceraldehyde-3-phosphate dehydrogenase | NM_008084.3 | F: TGCACCACCAACTGCTTAG R: GGATGCAGGGATGATGTTC |
177 | 94.2 | 0.996 |
| Grcc10 | Gene rich cluster, C10 gene | NM_013535.1 | F: GCGGAGGTGATTCAAGCG R: TGACCAGGCGGGCAAACT |
196 | 96 | 0.995 |
| Eif4h | Eukaryotic translation initiation factor 4H | NM_033561.2 | F: CCTTGGCTCGGGATTGTC R: TCCGCATTGGAGATGGATTA |
198 | 97.2 | 0.998 |
| Rnf187 | Ring finger protein 187 | NM_022423.2 | F: CTGGCACCACCCTTCATC R: ACAAGCCCGAGCACATTC |
155 | 95.1 | 0.991 |
| Ywhae | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein | NM_009536.4 | F: CCCATTCGTTTAGGTCTT R: TCCACAGCGTCAGGTTAT |
193 | 93.2 | 0.994 |
| Nedd8 | Neural precursor cell expressed, developmentally downregulated gene 8 | NM_008683.3 | F: TGGGAAGGAGATTGAGATAG R: TTGCTTGCCACTGTAGATG |
121 | 94 | 0.995 |
| 18S rRNA | 18S ribosomal RNA | NR_003278.2 | F: TTGACGGAAGGGCACCACCAG R: GCACCACCACCCACGGAATCG |
130 | 91.7 | 0.996 |
| Rpl13 | Ribosomal protein L-13 | NM_016738.5 | F: GTACGCTGTGAAGGCATCAA R: ATCCCATCCAACACCTTGAG |
135 | 91 | 0.997 |
| Ubc | Ubiquitin C | NM_019639 | F: GCCCAGTGTTACCACCAAGA R: CCCATCACACCCAAGAACA |
104 | 96.3 | 0.996 |
| Rpl32 | Ribosomal protein L32 | NM_172086.2 | F: GAACTGGCGGAAACCCA R: GGATCTGGCCCTTGAACCTT |
63 | 96.2 | 0.995 |
| Ppia | Peptide acyl preserved ammonia acyl isomerase A | NM_008907.1 | F: CGCTTGCTGCAGCCATGGTC R: CAGCTCGAAGGAGACGCGGC |
86 | 96.3 | 0.990 |
The correlation coefficients (R2) of the standard curve, efficiency of each pair of primers, length of the RT-qPCR transcripts, and primer sequences are indicated. RT-qPCR: reverse transcription-quantitative polymerase chain reaction.