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. Author manuscript; available in PMC: 2020 May 6.
Published in final edited form as: J Vis Exp. 2019 Apr 5;(146):10.3791/59400. doi: 10.3791/59400

Figure 1: Validation of HL-60 cell differentiation via flow cytometry.

Figure 1:

Differentiated HL-60 cells were harvested, washed, and resuspended in 1 × 105 cells/mL PBS. Cells were then aliquoted into 12 wells (100 μL/well) in a 96-well plate. Cells were then stained with fluorescently conjugated anti-CD35, anti-CD71, annexin V, and propidium iodide. Unstained cells or cells stained with fluorescently conjugated isotype antibodies were used as controls.