Figure 2.

Effects of BHD, mBHD-1, and mBHD-2 on NO production in LPS-exposed RAW264.7 cells. Cells were pretreated with different concentrations of BHD (a), mBHD-1 (b), and mBHD-2 (c) for 1 h and then were stimulated with 100 ng/mL LPS. After 24 h, secreted NO was measured in conditioned media using a NO detection kit. ^∗∗∗p < 0.001 vs. LPS. Statistical differences were analyzed using one-way ANOVA followed by Tukey's multiple comparisons test. Error bars indicate SEM.