Figure 2.

In vitro adherence and viability of hMSCs. The adherence and viability of hMSCs on Gelfoam® was evaluated using DiI imaging (a) and MTS assay (b). Representative image shows the red cytoplasmic fluorescence of hMSCs adhered to Gelfoam® after 6 days. A portion of the image is out of focus because of the 3D nature of the scaffold. Proliferation of hMSCs on Gelfoam® is comparable to cells seeded on tissue culture polystyrene surface for 2, 4, and 6 days. The absorbance at 490 nm is directly proportional to the number of living and proliferating cells. Tissue culture surface and Gelfoam® without any cells in the same culture conditions were used as blanks to obtain normalized values at each time point.