Figure 2.
lncRNA IGHCγ1 promoted macrophage proliferation and migration. (a) Real-time PCR showed lncRNA IGHCγ1 expression after overexpression in macrophages (N = 3; ∗∗∗P < 0.001). (b) Real-time PCR presented IGHCγ1 expression after knockdown in macrophages (N = 3; ∗∗∗P < 0.001). (c) CCK-8 revealed that lncRNA IGHCγ1 enhanced the proliferation of macrophages (N = 3; ∗P < 0.05; ∗∗P < 0.01). (d) siRNA of IGHCγ1 inhibited macrophage proliferation assayed by CCK-8 (N = 3; compared with the control group, ∗P < 0.05; ∗∗P < 0.01). (e) IGHCγ1 promoted macrophage proliferation when it was upregulated in cells assayed by EdU (representative pictures of EdU assays). (f) lncRNA IGHCγ1 promoted macrophage proliferation (data of three repeated EdU experiments; compared with controls, ∗∗P < 0.01).