Skip to main content
. 2020 Jan 17;2020:9743037. doi: 10.1155/2020/9743037

Figure 3.

Figure 3

lncRNA IGHCγ1 functioned as ceRNA via binding miR-6891-3p. (a) Real-time PCR revealed lncRNA IGHCγ1 primarily existed in the cytoplasm of macrophages (these data represented 3 independent experiments; compared with controls, ∗∗∗P < 0.001; compared with the LPS-treated macrophage group, ###P < 0.001). (b) FISH also showed lncRNA IGHCγ1 was mainly expressed in pTHP-1 cytoplasm (pictures represent one of three repeated FISH assays). (c) Increased copy number gains of lncRNA IGHCγ1 in OA PBMC samples (N = 32). (d) Increased copy number gains of lncRNA IGHCγ1 relative to miR-6891-3p in pTHP-1 cells stimulated by LPS (N = 3; ∗P < 0.05; ∗∗P < 0.01). (e) As shown by real-time PCR, the expression of miR-6891-3p in macrophages was significantly reduced when lncRNA IGHCγ1 was overexpressed (N = 3; ∗∗P < 0.01). (f) The seed sequence of miR-6891-3p recognized by lncRNA IGHCγ1 (data were screened in database of starBase). (g) Decreased luciferase activity in lncRNA IGHCγ1 WT transfected cells but not lncRNA IGHCγ1 MT cells (N = 3; ∗∗∗P < 0.001). (h) RIP assay showed IGHCγ1 in immunoprecipitates. Cell lysates were immunoprecipitated by use of Ago2 antibody and IgG. IGHCγ1 expression is determined by real-time PCR (N = 3; ∗∗P < 0.01).