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. 2020 Jan 17;2020:9743037. doi: 10.1155/2020/9743037

Figure 7.

Figure 7

lncRNA IGHCγ1 functioned as a ceRNA for miR-6891-3p through NF-κB signaling. (a) lncRNA IGHCγ1 promoted NF-κB phosphorylation by sponging miR-6891-3p, while miR-6891-3p mimics rescued its inhibitory effect on NF-κB phosphorylation in macrophages (representative western blot assay). (b) The densitometry of p-NF-κB determined by western blot (N = 3; in contrast to the -/- group, ∗∗P < 0.01; compared with the +/- group, #P < 0.05). (c) lncRNA IGHCγ1 increased NF-κB activity by acting as ceRNA for miR-6891-3p, whereas miR-6891-3p mimics rescued its effect on NF-κB in pTHP-1 cells (N = 3; ∗∗∗P < 0.001). (d) Expression of IL-6 and TNF-α mRNAs in macrophages (N = 3; compared with the control (-/-) group, ∗∗P < 0.01; in contrast to the lncRNA/mimics (+/-) group, #P < 0.05; ##P < 0.01). (e) IL-6 and TNF-α in the supernatant of macrophages (N = 3; in contrast to the control (-/-) group, ∗∗∗P < 0.001; compared with the lncRNA/mimics (+/-) group, ##P < 0.01).