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. 2020 Jan 11;2020:7126914. doi: 10.1155/2020/7126914

Figure 5.

Figure 5

Intercellular ROS accumulation was relieved by PUR in human NPMSCs under compression via the PI3K/Akt pathway. (a) Representative fluorescence imaging of ROS production in human NPMSCs loaded with DCFDA (scale bar = 100 μm). (b) Quantitative analysis of ROS fluorescence staining. (c) Histogram analysis of ROS flow cytometry, which represented the mean fluorescence intensity (the average intercellular ROS accumulation) in NPMSCs in each group. (d) Detection of intercellular ROS production by flow cytometry. Data are presented as the means ± SD (n = 3) (P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 between two groups). ROS: reactive oxygen species; PUR: puerarin.