Table 1. Summary of primers and restriction enzymes for SNP genotyping.
SNP | Forward primer (5′–3′) | Reverse primer (5′–3′) | Amplicon size | Restriction enzyme | Reference |
---|---|---|---|---|---|
COX-2 -765 C>G | AGGCAGGAAACTTTATATTGG | ATGTTTTAGTGACGACGCTTA | 309 | SsiI | Shin et al. (2012) [13] |
COX-2 -1195 A>G | CCCTGAGCACTACCCATGAT | GCCCTTCATAGGAGATACTGG | 273 | PvuII | Shin et al. (2012) [13] |
EGF +61 A>G | TGTCACTAAAGGAAAGGAGGT | TTCACAGAGTTTAACAGCCC | 242 | AluI | Shahbazi et al. (2002) [27] |
EGFR R521K G>A | TGCTGTGACCCACTCTGTCT | CCAGAAGGTTGCACTTGTCC | 155 | MvaI | Sasaki et al. (2009) [28] |
TP53 P72R C>G | AATGGATGATTTGATGCTGTCCC | CGTGCAAGTCACAGACTTGGC | 259 | BstUI | Ueda et al. (2005) [29] |