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. 2020 May 5;40(5):BSR20191188. doi: 10.1042/BSR20191188

Table 1. Summary of primers and restriction enzymes for SNP genotyping.

SNP Forward primer (5′–3′) Reverse primer (5′–3′) Amplicon size Restriction enzyme Reference
COX-2 -765 C>G AGGCAGGAAACTTTATATTGG ATGTTTTAGTGACGACGCTTA 309 SsiI Shin et al. (2012) [13]
COX-2 -1195 A>G CCCTGAGCACTACCCATGAT GCCCTTCATAGGAGATACTGG 273 PvuII Shin et al. (2012) [13]
EGF +61 A>G TGTCACTAAAGGAAAGGAGGT TTCACAGAGTTTAACAGCCC 242 AluI Shahbazi et al. (2002) [27]
EGFR R521K G>A TGCTGTGACCCACTCTGTCT CCAGAAGGTTGCACTTGTCC 155 MvaI Sasaki et al. (2009) [28]
TP53 P72R C>G AATGGATGATTTGATGCTGTCCC CGTGCAAGTCACAGACTTGGC 259 BstUI Ueda et al. (2005) [29]