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. 2020 May 5;40(5):BSR20200378. doi: 10.1042/BSR20200378

Figure 2. miR-202-3p inhibits sST2 expression induced by Ang II.

Figure 2

Human macrophage THP-1 cells were seeded into six-well plate and starved overnight. After that, cells were treated with different concentrations of Ang II (0, 0.1, 1 and 10 μM) for 24 h, respectively. After that, cells were harvested to analyze the expression of sST2 (A) and miR-202-3p (B). The mRNA levels were normalized to β-ACTIN mRNA and the levels of miRNA were normalized to U6b. All data were expressed as mean ± SD and the differences of gene expression between groups treated with different doses of ang II were examined by Student’s t test. *P<0.05, **P<0.01, ***P<0.005 vs untreated control. (C) THP-1 cells were seeded into six-well plate and transfected with miR-202-3p mimic or negative control mimic (NC). At 24 h post transfected, cells were further stimulated with 10 μM Ang II for another 24 h. After that, cells were collected to analyze the expression of sST2. The difference of gene expression between groups transfected with miR-202-3p and negative control was examined by Student’s t test. **P<0.01 vs untreated control, *P<0.05 vs negative control mimic. All data represent three independent experiments.