Figure 5.

Phase-contrast photomicrographs of differentiated NSC-34 cells treated with PGE2 and EP agonists. Differentiated NSC-34 cells were treated with vehicle (0.15% DMSO), 80 μM PGE2, 40 μM butaprost (an EP2 agonist), or 40 μM sulprostone (an EP1/3 agonist) in the presence or absence of NAC for 48 h. Cell morphology was observed using a phase-contrast microscope (IX71, Olympus, Tokyo, Japan). Photographs show typical phase-contrast microscopy images in each treatment group. Scale bar indicates 50 μm.