Table 3.

Metabolite annotation and pathway enrichment analysis using Mummichog for negative ion m/z features present in the native and allograft BAL fluid. * P < 0.05, Native vs Allograft samples with less enrichment of these pathways. Fischer’s Exact test was used for comparison and generation of enrichment P values. P-values for all pathways were then modeled as a Gamma distribution and then adjusted for the permutations

Pathways	Pathway Size	Adjusted P Value
Purine Metabolism	22	0.001
Methionine and Cysteine Metabolism	13	0.002
Denovo-Fatty acid biosynthesis	10	0.002
Fatty acid activation	7	0.003
Vitamin B5-CoA biosynthesis	7	0.003
Aspartate and Aspargine Metabolism	21	0.003
Alanine and Aspartate Metabolism	5	0.005
Arginine and Proline Metabolism	9	0.006
Drug Metabolism CYP 450	33	0.008
Saturated fatty acid beta-oxidation	2	0.008
Phosphatidylinositol Phosphate Metabolism	6	0.009
TCA Cycle	3	0.01
Alkaloid Biosynthesis II	3	0.01
Urea cycle	20	0.01
Pyrimidine Metabolism	13	0.03
Dynorphin Metabolism	4	0.03
Omega 3 Fatty acid Metabolism	4	0.03
COA Metabolism	4	0.03
Fatty acid Metabolism	4	0.03
Vitamin E Metabolism	27	0.03
Linoleate Metabolism	9	0.03