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. 2020 May 6;11:29. doi: 10.1186/s13229-020-00340-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Intact miniature excitatory and inhibitory synaptic transmission in pyramidal cells and Nkx2.1 interneurons. a, b Representative traces (a) and graphs of cumulative probability (b) with group data (insets) showing unchanged mEPSC amplitude and frequency (or IEI, inter-event interval) in pyramidal cells of Nkx2.1^Cre/wt;Tsc1^f/wt mutant relative to control mice (n = 8 cells in Wt (Nkx2.1^wt/wt;Tsc1^f/wt) and 9 cells in Tg (Nkx2.1^Cre/wt;Tsc1^f/wt) mice; Kolmogorov-Smirnov tests for cumulative plots, Student’s t test for group data, p > 0.05). c, d Representative traces (c) and graphs of cumulative probability (d) with group data (insets) showing unchanged mIPSC amplitude and frequency (or IEI) in pyramidal cells of Nkx2.1^Cre/wt;Tsc1^f/wt mutant relative to control mice (n = 10 cells in Wt (Nkx2.1^wt/wt;Tsc1^f/wt) and 14 cells in Tg (Nkx2.1^Cre/wt;Tsc1^f/wt) mice; Kolmogorov-Smirnov tests and Student’s t tests, p > 0.05). e, f Fluorescence images at low (e) and high (f) magnification showing the presence (right) of EYFP-positive Nkx2.1 cells in Nkx2.1^Cre/wt mouse injected with AAV2/9-DIO-EYFP in CA1 hippocampus and the absence of EYFP expression after similar injection in control Tsc1^f/f mice. Scale bars in images at right apply also to images at left. g–j Similar electrophysiological data representation showing unchanged mEPSCs (g–h) and mIPSCs (i, j) in EYFP-expressing Nkx2.1 interneurons of Nkx2.1^Cre/wt;Tsc1^f/wt mutant relative to control mice (n = 13 cells in Wt (Nkx2.1^Cre/wt;Tsc1^wt/wt) and 9 cells in Tg (Nkx2.1^Cre/wt;Tsc1^f/wt) mice; Kolmogorov-Smirnov tests and Student’s t tests, p > 0.05). Note that in (g–j) mEPSCs and mIPSCs were recorded in the same interneurons at − 70 and 0 mV, respectively, resulting in outward mIPSCs (see the “Methods” section)