Fig. 2.

miR-153 directly targets Jagged1 and suppresses the Notch activity in lung cancer cells. a mRNA expression of indicated genes involved in CSC pathways detected by qPCR. b Expression of Jagged1, NCID, and Notch target gene Hes1 were determined by Western blot. c Diagram of predicted binding sites of miR-153 on the 3′-UTR of Jagged1 gene. d Diagram of JAG1 3′-UTR wild-type and mutant reporter construct. e Luciferase reporter assay was performed in 293 T cells with co-transfection of indicated wild-type or mutant 3′-UTR constructs and miR-153 mimic. f Jagged1 expression was determined by immunofluorescence. Scale bar, 50 μm. Data shown are mean ± s.d. of three independent experiments. *P < 0.05 by two-tailed Student’s t test