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. 2020 May 6;10:14. doi: 10.1186/s13395-020-00231-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Skeletal muscle PGC-1alpha has little effect on whole-body metabolism in 24-month-old mice. a Glucose tolerance test (GTT) in male and female 24-month-old mice. Calculated AUC of GTTs. b Western blots for pAkt and Akt in quadriceps (quantification is normalized to loading control 14-3-3). c VO2, VCO2, and respiratory quotient (RER) in 24-month-old mice measured via Comprehensive Laboratory Animal Monitoring System (CLAMS). d Physical activity of 24-month-old mice measured via CLAMS [n = 5, 5 for 24-month-old WT and MCKa]. *P < 0.05 compared to control