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. 2020 May 5;21:25. doi: 10.1186/s12865-020-00350-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — BC-FAST and PCV treated mice control primary tumor growth. a Percentage of tumor free mice. Female Balb/c mice (n = 10/group) were challenged, vaccinated and tumor volume was measured twice per week. Mice were vaccinated with PBS (Mock), BC-PCV, BC-FAST, PC-FAST, or NR-PCV. Data are presented as percentage of tumor free mice and p values calculated using Log-rank (Mantel-Cox) Test. b Average 4 T1 tumor growth curves. The tumor volume of 4T1-tumor bearing mice was measured and plotted for BC-PCV, BC-FAST, or Mock vaccinated mice (b) and for PC-FAST and NR-PCV vaccinated mice (d). c Kaplan-Meyer survival curves of vaccinated mice. At 47–55 days post challenge, mice with tumor volume < 1000 mm³ and/or no clinical signs of illness (weight loss, skin ulceration, hair loss or lethargy) were considered survivors. Data were analyzed using Log-rank (Mantel-Cox) Test (*** p < 0.001)