Fig. 6.
FS vaccinated mice have T-cells that recognize 4 T1 tumor cells. IFN-γ positive splenocytes stimulated by 4 T1 cells from (a) BC-FAST or BC-PCV treated mice, Mouse splenocytes were stimulated, in triplicate, with 4 T1 cells (1 × 105 cells/well) for 72 h and evaluated by IFN-γ ELISPOT. IFN-γ positive splenocytes stimulated by 4 T1 cells from a BC-FAST or BC-PCV mice (a) without and (b) with ICI, or (c) PC-FAST treated mice with or without ICI treatment. Bars represent mean ± S.E for each group and dot represents mouse individual immune response. Pooled mock splenocytes were tested in two independent experiments, and dots represent each replicate and bars mean ± S.E of these experiments. * p < 0.05; **, p < 0.001, analyzed by unpaired t test with Welch’s correction