Fig. 7.

BC-FAST vaccinated mice have functional CD4+ and CD8+ T cells. Multiparameter flow cytometry was used to determine the percentages of the cytokine generation in both CD8+ and CD4+ T cell of mice immunized with BC-FAST vaccine alone or in combination with ICIs. Naïve mice and Mock vaccinated groups are indicated at the top. Pie charts show the relative proportion for each tested cytokine population as well the Boolean combination gates for IFN-γ +, TNF-α +, IL-2+, GZB + and PD-1+. Arc charts shows the production of each individual cytokine/ surface marker. Assays were performed with cryopreserved cells without any prior in vitro expansion. Data is representative of average of cytokine production percentage from: Naïve = 3 mice; Mock, BC-FAST and BC-FAST + ICI = 5 mice each group